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ProductsBack/Goat anti-Ankyrin 1 / ANK1 Antibody

Goat anti-Ankyrin 1 / ANK1 Antibody

EB08509
Everest Biotech
ApplicationsELISA
Product group Antibodies
ReactivityBovine, Human, Mouse, Rat
TargetANK1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Ankyrin 1 / ANK1 Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA
  • Applications Supplier
    Pep-ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID286
  • Target name
    ANK1
  • Target description
    ankyrin 1
  • Target synonyms
    ANK, SPH1, SPH2, ankyrin-1, ankyrin-1, ANK-1, ankyrin 1, erythrocytic, ankyrin-R, erythrocyte ankyrin
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000028.3; NP_065208.2; NP_065209.2; NP_065210.2; NP_065211.2; NP_065213.2. GeneIDs all Nonhuman: 11733 (mouse); 306570 (rat);. Purification: Antigen affinity purified. Names and symbols: ANK1 ; ankyrin 1, erythrocytic ; ANK; SPH1; SPH2; ankyrin 1; ankyrin-1, erythrocytic; ankyrin-R
  • Reactivity
    Bovine, Human, Mouse, Rat
  • Reactivity Supplier
    Human, Mouse, Rat, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of Ankyrin Erythroid/ANK/ANK1 using anti-Ankyrin Erythroid/ANK/ANK1 antibody (A02716-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ankyrin Erythroid/ANK/ANK1 antigen affinity purified polyclonal antibody (Catalog # A02716-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ankyrin Erythroid/ANK/ANK1 at approximately 250 kDa. The expected band size for Ankyrin Erythroid/ANK/ANK1 is at 206 kDa.
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