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Goat anti-APOA1

EB08746
Everest Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetAPOA1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-APOA1 Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID335
  • Target name
    APOA1
  • Target description
    apolipoprotein A1
  • Target synonyms
    AMYLD3, HPALP2, apo(a), apolipoprotein A-I, apo-AI, epididymis secretory sperm binding protein
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000030.1. Purification: Antigen affinity purified. Names and symbols: APOA1; apolipoprotein A-I; MGC117399; amyloidosis; apolipoprotein A-I, preproprotein; apolipoprotein A1; preproapolipoprotein
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of APOA1 using anti-APOA1 antibody (PB9916). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOA1 antigen affinity purified polyclonal antibody (Catalog # PB9916) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOA1 at approximately 26 kDa. The expected band size for APOA1 is at 31 kDa.
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