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Goat anti-ATF6

EB05245
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
Product group Antibodies
TargetATF6
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ATF6 Antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of A549 and in the endoplasmic reticulum and cytoplasm of HeLa cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID22926
  • Target name
    ATF6
  • Target description
    activating transcription factor 6
  • Target synonyms
    ACHM7; ATF6A; cAMP-dependent transcription factor ATF-6 alpha; cyclic AMP-dependent transcription factor ATF-6 alpha
  • Host
    Goat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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U251 cells were stained with ATF6 Polyclonal Antibody (bs-1634R) at 1:200 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) FITC conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
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Figure 1. Western blot analysis of ATF6 using anti-ATF6 antibody (A00655-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF6 antigen affinity purified polyclonal antibody (Catalog # A00655-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATF6 at approximately 90-100 kDa. The expected band size for ATF6 is at 75 kDa.
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IHC image of CSB-PA002277LA01HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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