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Goat anti-C20orf75

EB08262
Everest Biotech
ApplicationsELISA
Product group Antibodies
TargetLRRN4
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-C20orf75 Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA
  • Applications Supplier
    Pep-ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID164312
  • Target name
    LRRN4
  • Target description
    leucine rich repeat neuronal 4
  • Target synonyms
    C20orf75, NLRR-4, NLRR4, dJ1056H1.1, leucine-rich repeat neuronal protein 4, neuronal leucine-rich repeat protein 4
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_689824.1. Purification: Antigen affinity purified. Names and symbols: LRRN4; C20orf75; chromosome 20 open reading frame 75; RP5-967N21.9; FLJ23994; MGC25027; NLRR4; dJ1056H1.1; hypothetical protein LOC164312; neuronal leucine-rich repeat protein 4
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LRRN4 using anti-LRRN4 antibody (A15151-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRN4 antigen affinity purified polyclonal antibody (Catalog # A15151-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRRN4 at approximately 79 kDa. The expected band size for LRRN4 is at 79 kDa.
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