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Goat anti-CBFB

EB12158
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCBFB
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-CBFB Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID865
  • Target name
    CBFB
  • Target description
    core-binding factor subunit beta
  • Target synonyms
    CLCD2, PEBP2B, core-binding factor subunit beta, CBF-beta, PEA2-beta, PEBP2-beta, SL3-3 enhancer factor 1 beta subunit, SL3-3 enhancer factor 1 subunit beta, SL3/AKV core-binding factor beta subunit, core-binding factor beta subunit, polyomavirus enhancer binding protein 2, beta subunit
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_074036.1; NP_001746.1. GeneIDs all Nonhuman: 12400 (mouse);. Purification: Antigen affinity purified. Names and symbols: CBFB; core-binding factor, beta subunit; PEBP2B; CBF-beta; PEA2-beta; PEBP2-beta; SL3-3 enhancer factor 1 beta subunit; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit; core-binding factor subunit beta; polyomavirus enhancer
  • Reactivity Supplier
    Human, Mouse, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of CBFb using anti-CBFb antibody (A01007-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBFb antigen affinity purified polyclonal antibody (Catalog # A01007-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBFb at approximately 22KD. The expected band size for CBFb is at 22KD.
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