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ProductsBack/Goat anti-Cd97 (mouse)

Goat anti-Cd97 (mouse)

EB11876
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetAdgre5
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Cd97 (mouse) Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID26364
  • Target name
    Adgre5
  • Target description
    adhesion G protein-coupled receptor E5
  • Target synonyms
    AA409984; adhesion G protein-coupled receptor E5; Cd97; CD97 antigen; EGF-TM7 receptor; leukocyte antigen CD97; TM7LN1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_036055.2; NP_001156501.1; NP_001156502.1; NP_001156503.1. GeneIDs all Nonhuman: 26364 (mouse);. Purification: Antigen affinity purified. Names and symbols: Cd97; CD97 antigen; AA409984; TM7LN1; EGF-TM7 receptor; adhesion G protein-coupled receptor E5
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD97/Adgre5 using anti-CD97/Adgre5 antibody (A30392-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates, Lane 7: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD97/Adgre5 antigen affinity purified polyclonal antibody (Catalog # A30392-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD97/Adgre5 at approximately 92KD. The expected band size for CD97/Adgre5 is at 92KD.
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