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ProductsBack/Goat anti-Centromere protein F / mitosin

Goat anti-Centromere protein F / mitosin

EB05622
Everest Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCENPF
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Centromere protein F / mitosin Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID1063
  • Target name
    CENPF
  • Target description
    centromere protein F
  • Target synonyms
    AH antigen; cell-cycle-dependent 350K nuclear protein; CENF; CENP-F kinetochore protein; centromere protein F; centromere protein F, 350/400kDa; CILD31; hcp-1; kinetochore protein CENPF; mitosin; PRO1779; STROMS
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): AAA82889.1; AAA82935.1; AAA86889.1. Purification: Antigen affinity purified. Names and symbols: CENPF; centromere protein F (350/400kD, mitosin); CENF; PRO1779; mitosin; AH antigen; CENP-F kinetochore protein; centromere protein F (400kD); cell-cycle-dependent 350K nuclear protein; centromere protein F, 350/400ka (mitosin); RP11-262H5.1; hcp-1; cent
  • Reactivity Supplier
    Human, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CENPF using anti-CENPF antibody (A03311-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CENPF antigen affinity purified polyclonal antibody (Catalog # A03311-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CENPF at approximately 358 kDa. The expected band size for CENPF is at 358 kDa.
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