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Goat anti-CGI58 / ABHD5

EB07657
Everest Biotech
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetABHD5
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-CGI58 / ABHD5 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm/ vesicles of U2OS cells. Recommended concentration: 10microg/ml.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IF, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID51099
  • Target name
    ABHD5
  • Target description
    abhydrolase domain containing 5, lysophosphatidic acid acyltransferase
  • Target synonyms
    CGI58, IECN2, NCIE2, 1-acylglycerol-3-phosphate O-acyltransferase ABHD5, abhydrolase domain-containing protein 5, lipid droplet-binding protein CGI-58
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_057090.2. GeneIDs all Nonhuman: 67469 (mouse); 316122 (rat);. Purification: Antigen affinity purified. Names and symbols: ABHD5; CGI58 protein; aCGI58; bhydrolase domain containing 5; CDS; IECN2; MGC8731; NCIE2
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Koltes DA, Spurlock DM. Coordination of lipid droplet-associated proteins during the transition period of Holstein dairy cows. J Dairy Sci. 2011,94(4):1839-48. doi: 10.3168/jds.2010-3769
    Read this paper

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Figure 1. Western blot analysis of Abhd5 using anti-Abhd5 antibody (PB10021). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: A431 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Abhd5 antigen affinity purified polyclonal antibody (Catalog # PB10021) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Abhd5 at approximately 43KD. The expected band size for Abhd5 is at 39KD.
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