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ProductsBack/Goat anti-Connexin 43 / GJA1

Goat anti-Connexin 43 / GJA1

EB09301
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetGJA1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Connexin 43 / GJA1 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID2697
  • Target name
    GJA1
  • Target description
    gap junction protein alpha 1
  • Target synonyms
    AVSD3; CMDR; connexin-43; CX43; EKVP; EKVP3; gap junction 43 kDa heart protein; gap junction alpha-1 protein; gap junction protein, alpha 1, 43kDa; GJAL; HLHS1; HSS; ODDD; PPKCA
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000156.1. GeneIDs all Nonhuman: 24392 (rat);. Purification: Antigen affinity purified. Names and symbols: GJA1; gap junction protein, alpha 1, 43kDa; CX43; DFNB38; GJAL; ODDD; connexin 43; gap junction 43 kDa heart protein; gap junction protein, alpha-like
  • Reactivity Supplier
    Human, Rat, Dog, Pig
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Analysis of the behavioral, cellular and molecular characteristics of pain in severe rodent spinal cord injury. Lee-Kubli CA et al., 2016 Apr, Exp Neurol
    Read more

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Figure 1. Western blot analysis of GJA1 using anti-GJA1 antibody (A00599). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse testicular tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GJA1 antigen affinity purified polyclonal antibody (Catalog # A00599) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GJA1 at approximately 43KD. The expected band size for GJA1 is at 43KD.
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