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ProductsBack/Goat anti-Coronin 1 / TACO

Goat anti-Coronin 1 / TACO

EB06057
Everest Biotech
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCORO1A
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Coronin 1 / TACO Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID11151
  • Target name
    CORO1A
  • Target description
    coronin 1A
  • Target synonyms
    CLABP; CLIPINA; clipin-A; coronin, actin binding protein, 1A; coronin-1; coronin-1A; coronin-like protein A; coronin-like protein p57; HCORO1; IMD8; p57; TACO; tryptophan aspartate-containing coat protein
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_009005.1. GeneIDs all Nonhuman: 12721 (mouse); 155151 (rat);. Purification: Antigen affinity purified. Names and symbols: CORO1A; Coronin 1; TACO; p57; CLABP; HCORO1; CLIPINA; Clabp TACO; coronin, actin binding protein, 1A; coronin-1; coronin, actin-binding, 1A; coronin, actin-binding protein, 1A; FLJ41407; MGC117380;
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Identification of differentially expressed proteins in spontaneous thymic lymphomas from knockout mice with deletion of p53. Honore B et al., 2008 Jun 10, Proteome Sci
    Read more

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Figure 1. Western blot analysis of Coronin 1a/TACO/CORO1A using anti-Coronin 1a/TACO/CORO1A antibody (A04245-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat spleen tissue lysates, Lane 3: rat thymus tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Coronin 1a/TACO/CORO1A antigen affinity purified polyclonal antibody (Catalog # A04245-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Coronin 1a/TACO/CORO1A at approximately 57KD. The expected band size for Coronin 1a/TACO/CORO1A is at 57KD.
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