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ProductsBack/Goat anti-CSX1 / NKX2-5

Goat anti-CSX1 / NKX2-5

EB09412
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNKX2-5
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-CSX1 / NKX2-5 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of U2OS cells. Recommended concentration: 10microg/ml.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IF, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID1482
  • Target name
    NKX2-5
  • Target description
    NK2 homeobox 5
  • Target synonyms
    CHNG5, CSX, CSX1, HLHS2, NKX2.5, NKX2E, NKX4-1, VSD3, homeobox protein Nkx-2.5, NK2 transcription factor related, locus 5, NKX 2-5, cardiac-specific homeobox 1, homeobox protein CSX, homeobox protein NK-2 homolog E, homeobox protein NKX 2-5, tinman homolog, tinman paralog
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_004378.1; NP_001159647.1; NP_001159648.1. GeneIDs all Nonhuman: 18091 (mouse); 114109 (rat);. Purification: Antigen affinity purified. Names and symbols: NKX2-5; NK2 transcription factor related, locus 5 (Drosophila); CHNG5; CSX; CSX1; NKX2.5; NKX2E; NKX4-1; NK2 transcription factor homolog E; NK2 transcription factor related, locus 5; cardiac-specific homeo box; tinman paralog
  • Reactivity Supplier
    Human, Mouse, Rat, Cow, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Nkx2.5/NKX2-5 using anti-Nkx2.5/NKX2-5 antibody (A00738-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nkx2.5/NKX2-5 antigen affinity purified polyclonal antibody (Catalog # A00738-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nkx2.5/NKX2-5 at approximately 35 kDa. The expected band size for Nkx2.5/NKX2-5 is at 35 kDa.
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