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Goat anti-DMAP1 Antibody, Affinity Purified

A300-217A
Bethyl Laboratories
ApplicationsWestern Blot
Product group Antibodies
TargetDMAP1
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Goat anti-DMAP1 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID55929
  • Target name
    DMAP1
  • Target description
    DNA methyltransferase 1 associated protein 1
  • Target synonyms
    DNA methyltransferase 1-associated protein 1; DNMAP1; DNMT1 associated protein 1; DNMTAP1; EAF2; MEAF2; SWC4
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ9NPF5
  • Protein Name
    DNA methyltransferase 1-associated protein 1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DMAP1 using anti-DMAP1 antibody (A03688-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat stomach tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DMAP1 antigen affinity purified polyclonal antibody (Catalog # A03688-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DMAP1 at approximately 53-60 kDa. The expected band size for DMAP1 is at 53 kDa.
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