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ProductsBack/Goat anti-EndoPDI / TXNDC5

Goat anti-EndoPDI / TXNDC5

EB06471
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTXNDC5
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-EndoPDI / TXNDC5 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum of U2OS and HeLa cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID81567
  • Target name
    TXNDC5
  • Target description
    thioredoxin domain containing 5
  • Target synonyms
    ENDOPDI, ERP46, HCC-2, HCC2, PDIA15, STRF8, UNQ364, thioredoxin domain-containing protein 5, ER protein 46, endoplasmic reticulum protein ERp46, endoplasmic reticulum resident protein 46, endothelial protein disulphide isomerase, epididymis secretory sperm binding protein, protein disulfide isomerase family A, member 15, thioredoxin domain containing 5 (endoplasmic reticulum), thioredoxin related protein, thioredoxin-like protein p46
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_110437.2; NP_001139021.1. Purification: Antigen affinity purified. Names and symbols: TXNDC5; EndoPDI; thioredoxin domain containing 5; ERP46; UNQ364; MGC3178; FLJ21353; FLJ90810; thioredoxin related protein; endothelial protein disulphide; thioredoxin domain containing 5 (endoplasmic reticulum) isomerase; Hcc-2; PDIA15; endothelial protei
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TXNDC5 using anti-TXNDC5 antibody (A05772-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TXNDC5 antigen affinity purified polyclonal antibody (Catalog # A05772-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TXNDC5 at approximately 50-55 kDa. The expected band size for TXNDC5 is at 48 kDa.
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