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Goat anti-ERCC1

EB08190
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetERCC1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ERCC1 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID2067
  • Target name
    ERCC1
  • Target description
    ERCC excision repair 1, endonuclease non-catalytic subunit
  • Target synonyms
    COFS4, RAD10, UV20, DNA excision repair protein ERCC-1, excision repair cross-complementation group 1, excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_973730.1; NP_001974.1. Purification: Antigen affinity purified. Names and symbols: ERCC1; excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence); COFS4; UV20; excision repair cross-complementing 1; excision repair protein
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Sikorski K, Mehta A, Inngjerdingen M, et al. A high-throughput pipeline for validation of antibodies. Nat Methods. 2018,15(11):909-912. doi: 10.1038/s41592-018-0179-8
    Read this paper

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Figure 1. Western blot analysis of ERCC1 using anti-ERCC1 antibody (A00388-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat stomach tissue lysates, Lane 2: mouse stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERCC1 antigen affinity purified polyclonal antibody (Catalog # A00388-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERCC1 at approximately 33KD. The expected band size for ERCC1 is at 33KD.
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