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ProductsBack/Goat anti-Fgf23 (mouse)

Goat anti-Fgf23 (mouse)

EB11045
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetFgf23
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Fgf23 (mouse) Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID64654
  • Target name
    Fgf23
  • Target description
    fibroblast growth factor 23
  • Target synonyms
    FGF-23; Fgf8b; fibroblast growth factor 23; fibroblast growth factor 8b
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_073148.1. GeneIDs all Nonhuman: 64654 (mouse); 170583 (rat);. Purification: Antigen affinity purified. Names and symbols: Fgf23; fibroblast growth factor 23; ADHR; HPDR2; HYPF; PHPTC; FGF-23; phosphatonin; tumor-derived hypophosphatemia inducing factor; tumor-derived hypophosphatemia-inducing factor
  • Reactivity Supplier
    Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FGF 23 using anti-FGF 23 antibody (PB9868). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF 23 antigen affinity purified polyclonal antibody (Catalog # PB9868) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF 23 at approximately 34 kDa. The expected band size for FGF 23 is at 28 kDa.
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ApplicationsWestern Blot
TargetFgf23
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