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Goat anti-FOXC1 (C Term)

EB06006
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
Product group Antibodies
TargetFOXC1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-FOXC1 (C Term) Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nucleus of U2OS cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA
  • Applications Supplier
    Pep-ELISA, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID2296
  • Target name
    FOXC1
  • Target description
    forkhead box C1
  • Target synonyms
    ARA; ASGD3; FKHL7; forkhead box C1 protein; forkhead box protein C1; forkhead, drosophila, homolog-like 7; forkhead/winged helix-like transcription factor 7; forkhead-related activator 3; forkhead-related protein FKHL7; forkhead-related transcription factor 3; FREAC3; FREAC-3; IGDA; IHG1; IRID1; myeloid factor-delta; RIEG3
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001444.2. GeneIDs all Nonhuman: 17300 (mouse);. Purification: Antigen affinity purified. Names and symbols: FOXC1; forkhead box C1; ARA; IGDA; IHG1; FKHL7; IRID1; FREAC3; iridogoniodysgenesis type 1; forkhead (Drosophila)-like 7; forkhead-related activator 3; Forkhead, drosophila, homolog-like 7; FREAC-3; RIEG3; forkhead/winged helix-like transcription factor 7
  • Reactivity Supplier
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FOXC1 using anti-FOXC1 antibody (A00962-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXC1 antigen affinity purified polyclonal antibody (Catalog # A00962-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXC1 at approximately 75KD. The expected band size for FOXC1 is at 57KD.
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