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ProductsBack/Goat anti-GAD1 (isoform GAD67)

Goat anti-GAD1 (isoform GAD67)

EB09109
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetGAD1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-GAD1 (isoform GAD67) Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in A431 cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID2571
  • Target name
    GAD1
  • Target description
    glutamate decarboxylase 1
  • Target synonyms
    67 kDa glutamic acid decarboxylase; CPSQ1; DEE89; GAD; GAD-67; glutamate decarboxylase 1; glutamate decarboxylase 1 (brain, 67kDa); SCP
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000808.2. GeneIDs all Nonhuman: 14415 (mouse); 24379 (rat);. Purification: Antigen affinity purified. Names and symbols: GAD1; glutamate decarboxylase 1 (brain, 67kDa); FLJ45882; GAD; SCP; OTTHUMP00000041055; glutamate decarboxylase 1; glutamate decarboxylase 1 (brain, 67kD); GAD67; GAD25
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow, Pig
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GAD67/GAD1 using anti-GAD67/GAD1 antibody (PB9183). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAD67/GAD1 antigen affinity purified polyclonal antibody (Catalog # PB9183) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAD67/GAD1 at approximately 67 kDa. The expected band size for GAD67/GAD1 is at 67 kDa.
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