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ProductsBack/Goat anti-GAPDH (Internal)

Goat anti-GAPDH (Internal)

EB07069
Everest Biotech
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetGAPDH
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-GAPDH (Internal) Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 and HeLa cells. Recommended concentration: 5-10microg/ml.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID2597
  • Target name
    GAPDH
  • Target description
    glyceraldehyde-3-phosphate dehydrogenase
  • Target synonyms
    G3PD, GAPD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, OCAS, p38 component, Oct1 coactivator in S phase, 38 Kd component, aging-associated gene 9 protein, epididymis secretory sperm binding protein Li 162eP, peptidyl-cysteine S-nitrosylase GAPDH
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_002037.2; NP_001243728.1. GeneIDs all Nonhuman: 14433 (mouse); 24383 (rat);. Purification: Antigen affinity purified. Names and symbols: GAPDH; glyceraldehyde-3-phosphate dehydrogenase; HGNC:4141; G3PD; GAPD; MGC88685; OTTHUMP00000174431; OTTHUMP00000174432; aging-associated gene 9 protein; glyceraldehyde 3-phosphate dehydrogenase
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • von Achenbach C, Silginer M, Blot V, et al. Depatuxizumab Mafodotin (ABT-414)-induced Glioblastoma Cell Death Requires EGFR Overexpression, but not EGFR(Y1068) Phosphorylation. Mol Cancer Ther. 2020,19(6):1328-1339. doi: 10.1158/1535-7163.MCT-19-0609
    Read this paper
  • Zheng J, He Q, Tang H, et al. miR-455-5p Overexpression Reduces Rat Lung Alveolar Type II Cell Proliferation by Downregulating STRA6. Anat Rec (Hoboken). 2019,302(11):2062-2069. doi: 10.1002/ar.24145
    Read this paper
  • Meier K, Jaguva Vasudevan AA, Zhang Z, et al. Equine MX2 is a restriction factor of equine infectious anemia virus (EIAV). Virology. 2018,523:52-63. doi: 10.1016/j.virol.2018.07.024
    Read this paper
  • von Achenbach C, Weller M, Szabo E. Epidermal growth factor receptor and ligand family expression and activity in glioblastoma. J Neurochem. 2018,147(1):99-109. doi: 10.1111/jnc.14538
    Read this paper
  • Happold C, Stojcheva N, Silginer M, et al. Transcriptional control of O(6) -methylguanine DNA methyltransferase expression and temozolomide resistance in glioblastoma. J Neurochem. 2018,144(6):780-790. doi: 10.1111/jnc.14326
    Read this paper
  • Ventura E, Weller M, Macnair W, et al. TGF-β induces oncofetal fibronectin that, in turn, modulates TGF-β superfamily signaling in endothelial cells. J Cell Sci. 2018,131(1):pii: jcs209619. doi: 10.1242/jcs.209619.
    Read this paper
  • Carr AJ, Vugler AA, Yu L, et al. The expression of retinal cell markers in human retinal pigment epithelial cells and their augmentation by the synthetic retinoid fenretinide. Mol Vis. 2011,17:1701-15.
    Read this paper
  • Carr AJ, Vugler A, Lawrence J, et al. Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay. Mol Vis. 2009,15:283-95.
    Read this paper
  • Vugler A, Carr AJ, Lawrence J, et al. Elucidating the phenomenon of HESC-derived RPE: anatomy of cell genesis, expansion and retinal transplantation. Exp Neurol. 2008,214(2):347-61. doi: 10.1016/j.expneurol.2008.09.007
    Read this paper

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Figure 1. Western blot analysis of GAPDH using anti-GAPDH antibody (A00227-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human CCRF-CEM whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAPDH antigen affinity purified polyclonal antibody (Catalog # A00227-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.
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