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ProductsBack/Goat anti-GNB3 & GNB4, Biotinylated

Goat anti-GNB3 & GNB4, Biotinylated

EB10456-B
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetGNB3
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-GNB3 & GNB4, Biotinylated Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Biotin
  • Gene ID2784
  • Target name
    GNB3
  • Target description
    G protein subunit beta 3
  • Target synonyms
    CSNB1H; G protein, beta-3 subunit; GTP-binding regulatory protein beta-3 chain; guanine nucleotide binding protein (G protein), beta polypeptide 3; guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 3; guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3; hypertension associated protein; transducin beta chain 3
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_002066.1, NP_067642.1. GeneIDs all Nonhuman: 60449 (rat);. Purification: Antigen affinity purified. Names and symbols: GNB3; guanine nucleotide binding protein (G protein), beta polypeptide 3; G protein, beta-3 subunit; GTP-binding regulatory protein beta-3 chain; guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 3; guanine nucleotide-binding protein, beta-3
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GNB3 using anti-GNB3 antibody (A02407-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCT tissue lysates, Lane 3: human HCCP tissue lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNB3 antigen affinity purified polyclonal antibody (Catalog # A02407-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GNB3 at approximately 37 kDa. The expected band size for GNB3 is at 37 kDa.
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