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ProductsBack/Goat anti-GP91-PHOX / NOX2

Goat anti-GP91-PHOX / NOX2

EB10581
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCYBB
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-GP91-PHOX / NOX2 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID1536
  • Target name
    CYBB
  • Target description
    cytochrome b-245 beta chain
  • Target synonyms
    AMCBX2, CGD, CGDX, GP91-1, GP91-PHOX, GP91PHOX, IMD34, NOX2, p91-PHOX, NADPH oxidase 2, CGD91-phox, cytochrome b(558) subunit beta, cytochrome b-245 beta polypeptide, cytochrome b-245 heavy chain, cytochrome b558 subunit beta, heme-binding membrane glycoprotein gp91phox, neutrophil cytochrome b 91 kDa polypeptide, p22 phagocyte B-cytochrome, superoxide-generating NADPH oxidase heavy chain subunit
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000388.2. GeneIDs all Nonhuman: 13058 (mouse); 66021 (rat);. Purification: Antigen affinity purified. Names and symbols: CYBB; cytochrome b-245, beta polypeptide; CGD; GP91-1; GP91-PHOX; GP91PHOX; NOX2; p91-PHOX; CGD91-phox; NADPH oxidase 2; cytochrome b(558) subunit beta; cytochrome b-245 heavy chain; cytochrome b558 subunit beta; heme-binding membrane glycoprotein gp91pho
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NOX2/gp91phox/CYBB using anti-NOX2/gp91phox/CYBB antibody (A00328-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human U937 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOX2/gp91phox/CYBB antigen affinity purified polyclonal antibody (Catalog # A00328-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOX2/gp91phox/CYBB at approximately 65KD. The expected band size for NOX2/gp91phox/CYBB is at 65KD.
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