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Goat anti-HE4 (rat)

EB12490
Everest Biotech
ApplicationsELISA
Product group Antibodies
TargetWfdc2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-HE4 (rat) Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA
  • Applications Supplier
    Pep-ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID286888
  • Target name
    Wfdc2
  • Target description
    WAP four-disulfide core domain 2
  • Target synonyms
    epididymal secretory protein 4; re4; WAP four-disulfide core domain protein 2
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_775132.1. GeneIDs all Nonhuman: 286888 (rat);. Purification: Antigen affinity purified. Names and symbols: Wfdc2; Re4
  • Reactivity Supplier
    Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HE4 using anti-HE4 antibody (A02685-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat thymus tissue lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse thymus tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HE4 antigen affinity purified polyclonal antibody (Catalog # A02685-3) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HE4 at approximately 19KD. The expected band size for HE4 is at 13KD.
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