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ProductsBack/Goat anti Human alpha-2 haptoglobin

Goat anti Human alpha-2 haptoglobin

GAHu/Hp
Nordic-MUbio
TargetHP
Product group Antibodies
1 ml
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Goat anti Human alpha-2 haptoglobin
  • Delivery Days Customer
    7
  • Application Supplier Note
    Precipitation assays. In immunoelectrophoresis use 2 microl or equivalent against 120 microl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 microl in a 3 mm diameter center well and 2 microl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID3240
  • Target name
    HP
  • Target description
    haptoglobin
  • Target synonyms
    binding peptide; BP; haptoglobin; haptoglobin alpha(1S)-beta; haptoglobin alpha(2FS)-beta; haptoglobin, alpha polypeptide; haptoglobin, beta polypeptide; HP2ALPHA2; HPA1S; zonulin
  • Host
    Goat
  • Protein IDP00738
  • Protein Name
    Haptoglobin
  • Source
    Haptoglobin is an acute phase protein synthesized in the liver. In immunoelectrophoresis it has an alpha-2 mobility. It forms a group of heterogeneous proteins consisting of four peptide chains, two alpha and two beta chains. The molecular weight is ...
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HP using anti-HP antibody (A00062-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HP antigen affinity purified polyclonal antibody (Catalog # A00062-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HP at approximately 45KD. The expected band size for HP is at 45KD.
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