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ProductsBack/Goat anti-Human Apo CI Antibody HRP Conjugated

Goat anti-Human Apo CI Antibody HRP Conjugated

A81-112P
Bethyl Laboratories
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetAPOC1

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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Goat anti-Human Apo CI Antibody HRP Conjugated
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    HRP
  • Estimated Purity
    ...
  • Gene ID341
  • Target name
    APOC1
  • Target description
    apolipoprotein C1
  • Target synonyms
    APOC1B, Apo-CI, ApoC-I, apo-CIB, apoC-IB, apolipoprotein C-I
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDP02654
  • Protein Name
    Apolipoprotein C-I
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Apolipoprotein CI using anti-Apolipoprotein CI antibody (M02526). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apolipoprotein CI antigen affinity purified monoclonal antibody (Catalog # M02526) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apolipoprotein CI at approximately 9 kDa. The expected band size for Apolipoprotein CI is at 9 kDa.
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