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ProductsBack/Goat anti-ILF2 / NF45

Goat anti-ILF2 / NF45

EB07784
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetILF2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ILF2 / NF45 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunoprecipitation: This antibody was confirmed by a customer in a special IP procedure called PAR-CLIP (see for method PMID: 20644507). This data is from a previous batch, not on sale.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID3608
  • Target name
    ILF2
  • Target description
    interleukin enhancer binding factor 2
  • Target synonyms
    NF45, PRO3063, interleukin enhancer-binding factor 2, interleukin enhancer binding factor 2, 45kD, interleukin enhancer binding factor 2, 45kDa, nuclear factor of activated T-cells, 45-kDa
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_004506.2; NP_001254738.1. GeneIDs all Nonhuman: 67781 (mouse); 310612 (rat);. Purification: Antigen affinity purified. Names and symbols: ILF2; NF45; interleukin enhancer binding factor 2, 45kDa ; MGC8391; PRO3063 ; interleukin enhancer binding factor 2; nuclear factor of activated T-cells, 45-kDa
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Ye J, Jin H, Pankov A, et al. NF45 and NF90/NF110 coordinately regulate ESC pluripotency and differentiation. RNA. 2017,23(8):1270-1284. doi: 10.1261/rna.061499.117
    Read this paper
  • Shamanna RA, Hoque M, Pe'ery T, et al. Induction of p53, p21 and apoptosis by silencing the NF90/NF45 complex in human papilloma virus-transformed cervical carcinoma cells. Oncogene. 2013,32(43):5176-85. doi: 10.1038/onc.2012.533
    Read this paper
  • Shamanna RA, Hoque M, Lewis-Antes A, et al. The NF90/NF45 complex participates in DNA break repair via nonhomologous end joining. Mol Cell Biol. 2011,31(23):4832-43. doi: 10.1128/MCB.05849-11
    Read this paper

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Figure 1. Western blot analysis of NF45/ILF2 using anti-NF45/ILF2 antibody (A04443-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF45/ILF2 antigen affinity purified polyclonal antibody (Catalog # A04443-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF45/ILF2 at approximately 43 kDa. The expected band size for NF45/ILF2 is at 43 kDa.
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