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ProductsBack/Goat anti-IRAK4 (N Terminus)

Goat anti-IRAK4 (N Terminus)

EB06619
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetIRAK4
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-IRAK4 (N Terminus) Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the cytoplasm and microtubules of HeLa cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID51135
  • Target name
    IRAK4
  • Target description
    interleukin 1 receptor associated kinase 4
  • Target synonyms
    IMD67; interleukin-1 receptor-associated kinase 4; IPD1; IRAK-4; NY-REN-64; REN64; renal carcinoma antigen NY-REN-64
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_057207.2; NP_001107654.1. GeneIDs all Nonhuman: 266632 (mouse);. Purification: Antigen affinity purified. Names and symbols: IRAK4; REN64; NY-REN-64; interleukin-1 receptor-associated kinase 4; IRAK-4 mutated form 1; interleukin-1 receptor associated kinase 4; interleukin-1 receptor associated kinase 4 mutant form 1; IPD1
  • Reactivity Supplier
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IRAK4 using anti-IRAK4 antibody (A01247-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRAK4 antigen affinity purified polyclonal antibody (Catalog # A01247-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRAK4 at approximately 52 kDa. The expected band size for IRAK4 is at 52 kDa.
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