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ProductsBack/Goat anti-KPNA2 / IPOA1, Biotinylated

Goat anti-KPNA2 / IPOA1, Biotinylated

EB06233-B
Everest Biotech
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetKPNA2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-KPNA2 / IPOA1, Biotinylated Antibody
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Biotin
  • Gene ID3838
  • Target name
    KPNA2
  • Target description
    karyopherin subunit alpha 2
  • Target synonyms
    IPOA1, PTAC58, QIP2, RCH1, SRP1-alpha, SRP1alpha, importin subunit alpha-1, RAG cohort protein 1, importin subunit alpha-2, importin-alpha-P1, karyopherin alpha 2 (RAG cohort 1, importin alpha 1), nuclear pore-targeting complex 58kD component, pendulin
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_002257.1. GeneIDs all Nonhuman: 16647 (mouse); 85245 (rat);. Purification: Antigen affinity purified. Names and symbols: KPNA2; karyopherin alpha 2 (RAG cohort 1, importin alpha 1); IPOA1; QIP2; RCH1; SRP1alpha; RAG cohort 1; RAG cohort protein 1; SRP1-alpha; importin alpha 1; importin alpha 2; importin subunit alpha-2; importin-alpha-P1; karyopherin subunit alpha-2; pendul
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KPNA2 using anti-KPNA2 antibody (A01776-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate,Lane 2: human HepG2 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KPNA2 antigen affinity purified polyclonal antibody (Catalog # A01776-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KPNA2 at approximately 58KD. The expected band size for KPNA2 is at 58KD.
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