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Goat anti-MAX

EB05058
Everest Biotech
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMAX
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-MAX Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Expression of the protein seen in the cytoplasm and nuclei of A431 and U251 cells. Recommended concentration: 10microg/ml. Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IF, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID4149
  • Target name
    MAX
  • Target description
    MYC associated factor X
  • Target synonyms
    PDMCS, bHLHd4, protein max, class D basic helix-loop-helix protein 4
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_002373.3; NP_660087.1. GeneIDs all Nonhuman: 17187 (mouse);. Purification: Antigen affinity purified. Names and symbols: MAX; MAX protein; MGC10775; MGC11225; MGC18164; MGC34679; MGC36767; myc-associated factor X; helix-loop-helix zipper protein; MYC associated factor X; bHLHd4; bHLHd5; bHLHd6; bHLHd7; bHLHd8; orf1
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Sikorski K, Mehta A, Inngjerdingen M, et al. A high-throughput pipeline for validation of antibodies. Nat Methods. 2018,15(11):909-912. doi: 10.1038/s41592-018-0179-8
    Read this paper

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Figure 1. Western blot analysis of MAX using anti-MAX antibody (A03239). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAX antigen affinity purified polyclonal antibody (Catalog # A03239) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAX at approximately 19-21 kDa. The expected band size for MAX is at 18 kDa.
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