Goat anti Monkey secretory component (free and bound)

Nordic-MUbio

Goat anti Monkey secretory component (free and bound)

7

Precipitation assays. In immunoelectrophoresis use 2 microl serum or equivalent against 120 microl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 microl antiserum in 3 mm diameter centre well and 2 microl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes. The antiserum reacts with both bound secretory component (secretory IgA) and with the free SC present in monkey secretions. In immunoelectrophoresis against monkey milk, using a high electroendosmosis agar plate, free SC is precipitated in the alph-2 region. The antiserum does not react with other molecular forms of IgA, or with any other secretory or plasma protein. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion to identify the presence secretory component in monkey serum or other body fluids and to determine its concentration.

ImmunoPrecipitation

Precipitation assays

Research Use Only

Polyclonal

Goat

Purified free secretory component isolated from pooled rhesus monkey milk is used for immunization. Freunds complete adjuvant is used in the first step of the immunization procedure.

Monkey

-20°C,2°C to 8°C

12352203