Goat anti Mouse C3c, conjugated with Biotin

Nordic-MUbio

Goat anti Mouse C3c, conjugated with Biotin

7

Biotin-conjugated IgG fraction of polyclonal goat antiserum to C3c fragment of mouse complement factor C3

ELISA, Immunocytochemistry, Immunohistochemistry (paraffin), Dot blot, Immunoblotting. In immunoelectrophoresis against fresh mouse serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c cab also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of mouse serum or plasma. The biotin immunoconjugate to mouse C3c is used to determine the presence and pattern of C3 in tissue lesions using immunohistochemical staining techniques. Locally deposited immune complexes in tissue usually contain complement, pointing to activation of the classical pathway. Complement activation in vivo implies active disease and may contribute to the elicitation of the pathogenesis and he extent of tissue destruction. In ELISA and Western blotting to identify mouse C3c in serum or other body fluids. As a second step an avidin or streptavidin conjugate of the users choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Dot Blot, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Dot blot;ELISA;Immunoblotting;Immunocytochemistry;Immunohistochemistry (paraffin)

Primary antibodies

Research Use Only

Polyclonal

Biotin

Goat

Mouse complement factor C3

The antiserum is raised against C3c, which is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. Consequently the antiserum reacts with both ...

Mouse

Mouse

12352203