Various whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
![E2F1 antibody [16G7] detects E2F1 protein at nucleus in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. E2F1 antibody [16G7] (GTX70163) diluted at 1:500. The signal was developed by Mouse IgG antibody (HRP) (GTX213111-01).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX213111-01/GTX213111-01_41421_20150909_IHC-P_M_w_23060620_535.webp "E2F1 antibody [16G7] detects E2F1 protein at nucleus in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. E2F1 antibody [16G7] (GTX70163) diluted at 1:500. The signal was developed by Mouse IgG antibody (HRP) (GTX213111-01).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Various whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Goat Anti-Mouse IgG antibody (HRP)
GTX213111-01
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityMouse
Overview
- SupplierGeneTex
- Product NameGoat Anti-Mouse IgG antibody (HRP)
- Delivery Days Customer9
- Application Supplier NoteWB: 1:5000-1:20000. IHC-P: 1:100-1:1000. ELISA: 1:5000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.25 mg/ml
- ConjugateHRP
- HostGoat
- IsotypeIgG
- Scientific DescriptionImmunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
- ReactivityMouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161