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Goat anti-NUDC

EB09516
Everest Biotech
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetNUDC
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-NUDC Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10microg/ml.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID10726
  • Target name
    NUDC
  • Target description
    nuclear distribution C, dynein complex regulator
  • Target synonyms
    HNUDC; MNUDC; NPD011; nuclear distribution C homolog; nuclear distribution gene C homolog; nuclear distribution protein C homolog; nuclear migration protein nudC; nudC nuclear distribution protein
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_006591.1. GeneIDs all Nonhuman: 18221 (mouse); 29648 (rat);. Purification: Antigen affinity purified. Names and symbols: NUDC; nuclear distribution gene C homolog (A. nidulans); HNUDC; MNUDC; NPD011; OTTHUMP00000004405; nuclear distribution gene C homolog
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NUDC using anti-NUDC antibody (A00995-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUDC antigen affinity purified polyclonal antibody (Catalog # A00995-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUDC at approximately 43 kDa. The expected band size for NUDC is at 43 kDa.
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