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Goat anti-Oxytocin Receptor

EB07183
Everest Biotech
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetOXTR
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Oxytocin Receptor Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, FC, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5021
  • Target name
    OXTR
  • Target description
    oxytocin receptor
  • Target synonyms
    OT-R, OTR, oxytocin receptor
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000907.2. GeneIDs all Nonhuman: 18430 (mouse); 25342 (rat);. Purification: Antigen affinity purified. Names and symbols: OXTR; oxytocin receptor; OT-R; OTR
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OXTR using anti-OXTR antibody (M01566). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OXTR antigen affinity purified monoclonal antibody (Catalog # M01566) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OXTR at approximately 43 kDa. The expected band size for OXTR is at 43 kDa.
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