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ProductsBack/Goat anti-PCK2 / PEPCK-M

Goat anti-PCK2 / PEPCK-M

EB06944
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetPCK2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-PCK2 / PEPCK-M Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5106
  • Target name
    PCK2
  • Target description
    phosphoenolpyruvate carboxykinase 2, mitochondrial
  • Target synonyms
    PEPCK, PEPCK-M, PEPCK2, mtPCK2, phosphoenolpyruvate carboxykinase [GTP], mitochondrial, PEP carboxykinase, epididymis secretory sperm binding protein, phosphopyruvate carboxylase
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_004554.3; NP_001278485.1. Purification: Antigen affinity purified. Names and symbols: PCK2; PEPCK-M; phosphoenolpyruvate carboxykinase 2 (mitochondrial); HGNC:8725; PEPCK; PEPCK2 ; PEP carboxykinase; mitochondrial phosphoenolpyruvate carboxykinase 2; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Méndez-Lucas A, Hyroššová P, Novellasdemunt L, et al. Mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) is a pro-survival, endoplasmic reticulum (ER) stress response gene involved in tumor cell adaptation to nutrient availability. J Biol Chem. 2014,289(32):22090-102. doi: 10.1074/jbc.M114.566927
    Read this paper
  • Méndez-Lucas A, Duarte JA, Sunny NE, et al. PEPCK-M expression in mouse liver potentiates, not replaces, PEPCK-C mediated gluconeogenesis. J Hepatol. 2013,59(1):105-13. doi: 10.1016/j.jhep.2013.02.020
    Read this paper

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Figure 1. Western blot analysis of PCK2 using anti-PCK2 antibody (A04772-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat spleen tissue lysates, Lane 10: rat kidney tissue lysates, Lane 11: rat liver tissue lysates, Lane 12: mouse brain tissue lysates, Lane 13: mouse kidney tissue lysates, Lane 14: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCK2 antigen affinity purified polyclonal antibody (Catalog # A04772-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCK2 at approximately 71KD. The expected band size for PCK2 is at 71KD.
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