More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Goat anti-PCK2 / PEPCK-M

Goat anti-PCK2 / PEPCK-M

EB06944
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetPCK2
100 ug
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-PCK2 / PEPCK-M Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5106
  • Target name
    PCK2
  • Target description
    phosphoenolpyruvate carboxykinase 2, mitochondrial
  • Target synonyms
    PEPCK, PEPCK-M, PEPCK2, mtPCK2, phosphoenolpyruvate carboxykinase [GTP], mitochondrial, PEP carboxykinase, epididymis secretory sperm binding protein, phosphopyruvate carboxylase
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_004554.3; NP_001278485.1. Purification: Antigen affinity purified. Names and symbols: PCK2; PEPCK-M; phosphoenolpyruvate carboxykinase 2 (mitochondrial); HGNC:8725; PEPCK; PEPCK2 ; PEP carboxykinase; mitochondrial phosphoenolpyruvate carboxykinase 2; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) is a pro-survival, endoplasmic reticulum (ER) stress response gene involved in tumor cell adaptation to nutrient availability. Mendez-Lucas A et al., 2014 Aug 8, J Biol Chem
    Read more
  • PEPCK-M expression in mouse liver potentiates, not replaces, PEPCK-C mediated gluconeogenesis. Mendez-Lucas A et al., 2013 Jul, J Hepatol
    Read more

FAQ

Go to our frequently asked questions

Related products

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA253485(PCK2 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: x200)
Product group Antibodies
Cusabio
PCK2 AntibodyCSB-PA253485
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetPCK2
  • SizePrice
Product group Antibodies
References
GeneTex
PCK2 antibody [C1C2], InternalGTX104663
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetPCK2
  • SizePrice
Product group Antibodies
MedChem Express
PCK2 Antibody (YA1547, Rabbit)HY-P81802
TargetPCK2
  • SizePrice
Figure 1. Western blot analysis of PCK2 using anti-PCK2 antibody (A04772-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat spleen tissue lysates, Lane 10: rat kidney tissue lysates, Lane 11: rat liver tissue lysates, Lane 12: mouse brain tissue lysates, Lane 13: mouse kidney tissue lysates, Lane 14: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCK2 antigen affinity purified polyclonal antibody (Catalog # A04772-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCK2 at approximately 71KD. The expected band size for PCK2 is at 71KD.
Product group Antibodies
Boster Bio
Anti-PCK2 Antibody Picoband(r)A04772-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetPCK2
  • SizePrice
Anti-PCK2 Antibody
Product group Antibodies
Antibodies.com
Anti-PCK2 AntibodyA38754
ApplicationsWestern Blot, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
RayBiotech
Anti-PCK2 Antibody144-08446
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
TargetPCK2
  • SizePrice
Lane 1: Mouse NIH/3T3 cell lysates; Lane 2: Mouse RAW264.7 cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human MCF-7 cell lysates; Lane 5: Human A431 cell lysates; Lane 6: Human Siha cell lysates probed with PI3KCA Polyclonal Antibody, Unconjugated (bs-5570R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
References
Bioss
phospho-PI3KCA (Tyr317) Polyclonal Antibodybs-5570R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetPCK2
  • SizePrice
Product group Antibodies
Biomatik
PCK2 Polyclonal AntibodyCAC14181
ApplicationsWestern Blot, ELISA
TargetPCK2
  • SizePrice
Product group Antibodies
Assay Genie
PEPCK/PCK2 AntibodyCAB8446
TargetPCK2
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PCK2 / PEPCK AntibodyLS-C401843
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetPCK2
  • SizePrice