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ProductsBack/Goat anti-PGAM1 / PGAM2 / PGAM4

Goat anti-PGAM1 / PGAM2 / PGAM4

EB05028
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPGAM1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-PGAM1 / PGAM2 / PGAM4 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5223
  • Target name
    PGAM1
  • Target description
    phosphoglycerate mutase 1
  • Target synonyms
    HEL-S-35, PGAM-B, PGAMA, phosphoglycerate mutase 1, BPG-dependent PGAM 1, epididymis secretory protein Li 35, phosphoglycerate mutase 1 (brain), phosphoglycerate mutase A, nonmuscle form, phosphoglycerate mutase isozyme B
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_002620.1; NP_000281.2; NP_001025062.1. Purification: Antigen affinity purified. Names and symbols: PGAM1; PGAM2; phosphoglycerate mutase 1 (brain); PGAMA; RP11-452K12.8; OTTHUMP00000059414; Phosphoglycerate mutase A, nonmuscle form; phosphoglycerate mutase 2 (muscle); MGC88743; PGAMM; Phosphoglycerate mutase, muscle form; PGAM3; phosphoglycerate mutas
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • A high-throughput pipeline for validation of antibodies. Sikorski K et al., 2018 Nov, Nat Methods
    Read more

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Figure 1. Western blot analysis of PGAM1 using anti-PGAM1 antibody (A04470-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGAM1 antigen affinity purified polyclonal antibody (Catalog # A04470-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGAM1 at approximately 29 kDa. The expected band size for PGAM1 is at 29 kDa.
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