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ProductsBack/Goat anti-PRDM4 / PFM1

Goat anti-PRDM4 / PFM1

EB06530
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPRDM4
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-PRDM4 / PFM1 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID11108
  • Target name
    PRDM4
  • Target description
    PR/SET domain 4
  • Target synonyms
    PFM1, PR domain zinc finger protein 4, PR domain 4, PR domain containing 4, PR domain-containing protein 4, PR-domain zinc-finger protein PFM1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_036538.3. Purification: Antigen affinity purified. Names and symbols: PRDM4; PFM1; MGC45046; PR domain containing 4; PR-domain zinc-finger protein PFM1; MGC45046
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Hela whole cell lysate, Mouse lung tissue, Mouse kidney tissue, Mouse skeletal muscle tissue All lanes: PRDM4 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 88 kDa Observed band size: 88, 70 kDa
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Figure 1. Western blot analysis of PRDM4 using anti-PRDM4 antibody (A11843-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human COLO320 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDM4 antigen affinity purified polyclonal antibody (Catalog # A11843-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRDM4 at approximately 88 kDa. The expected band size for PRDM4 is at 88 kDa.
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