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Goat anti-Prealbumin / Transthyretin

EB07248
Everest Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTTR
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Prealbumin / Transthyretin Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID7276
  • Target name
    TTR
  • Target description
    transthyretin
  • Target synonyms
    ATTR; CTS; CTS1; epididymis luminal protein 111; HEL111; HsT2651; PALB; prealbumin, amyloidosis type I; TBPA; thyroxine-binding prealbumin; transthyretin; TTN
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000362.1. GeneIDs all Nonhuman: 22139 (mouse); 24856 (rat);. Purification: Antigen affinity purified. Names and symbols: TTR; transthyretin (prealbumin, amyloidosis type I); HGNC:12405; HsT2651; PALB; TBPA; prealbumin; transthyretin
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Prealbumin using anti-Prealbumin antibody (PB9749). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysate, Lane 2: human HCCP tissue lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prealbumin antigen affinity purified polyclonal antibody (Catalog # PB9749) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prealbumin at approximately 14 kDa. The expected band size for Prealbumin is at 14 kDa.
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