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ProductsBack/Goat anti-prepro-transforming growth factor beta-2

Goat anti-prepro-transforming growth factor beta-2

EB13058
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetTGFB2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-prepro-transforming growth factor beta-2 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID7042
  • Target name
    TGFB2
  • Target description
    transforming growth factor beta 2
  • Target synonyms
    CAEND2, G-TSF, LDS4, TGF-beta2, transforming growth factor beta-2 proprotein, BSC-1 cell growth inhibitor, cetermin, glioblastoma-derived T-cell suppressor factor, polyergin, prepro-transforming growth factor beta-2
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001129071.1; NP_003229.1. Purification: Antigen affinity purified. Names and symbols: TGFB2; transforming growth factor beta 2; LDS4; TGF-beta2; BSC-1 cell growth inhibitor; G-TSF; cetermin; glioblastoma-derived T-cell suppressor factor; polyergin; prepro-transforming growth factor beta-2; transforming growth factor, beta 2
  • Reactivity Supplier
    Human, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TGF beta 2 using anti-TGF beta 2 antibody (A00892-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: huamn HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGF beta 2 antigen affinity purified polyclonal antibody (Catalog # A00892-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGF beta 2 at approximately 48 kDa. The expected band size for TGF beta 2 is at 48 kDa.
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