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Goat anti-PRPF31

EB07513
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetPRPF31
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-PRPF31 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HEK293 and HeLa cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID26121
  • Target name
    PRPF31
  • Target description
    pre-mRNA processing factor 31
  • Target synonyms
    NY-BR-99, PRP31, RP11, SNRNP61, U4/U6 small nuclear ribonucleoprotein Prp31, PRP31 pre-mRNA processing factor 31 homolog, U4/U6 snRNP 61 kDa protein, hPrp31, protein 61K, serologically defined breast cancer antigen NY-BR-99
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_056444.3. GeneIDs all Nonhuman: 68988 (mouse);. Purification: Antigen affinity purified. Names and symbols: PRPF31; PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae); DKFZp566J153; NY-BR-99; PRP31; RP11 ; PRP31 pre-mRNA processing factor 31 homolog (yeast); pre-mRNA processing factor 31 homolog; pre-mRNA processing factor 31 homolog (yeast)
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PRPF31 using anti-PRPF31 antibody (A03137-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRPF31 antigen affinity purified polyclonal antibody (Catalog # A03137-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRPF31 at approximately 60 kDa. The expected band size for PRPF31 is at 55 kDa.
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