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ProductsBack/Goat anti-Pycard (rodent)

Goat anti-Pycard (rodent)

EB12054
Everest Biotech
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
TargetPYCARD
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Pycard (rodent) Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    <Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3, KNRK and HeLa cells. Recommended concentration:10)microg/ml
  • Applications
    ImmunoFluorescence, ELISA
  • Applications Supplier
    Pep-ELISA, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID29108
  • Target name
    PYCARD
  • Target description
    PYD and CARD domain containing
  • Target synonyms
    ASC, CARD5, TMS, TMS-1, TMS1, apoptosis-associated speck-like protein containing a CARD, caspase recruitment domain-containing protein 5, target of methylation-induced silencing 1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_075747.3. GeneIDs all Nonhuman: 66824 (mouse); 282817 (rat);. Purification: Antigen affinity purified. Names and symbols: Pycard; PYD and CARD domain containing; 9130417A21Rik; Asc; CARD5; TMS-1; TNS1; masc; PYD and CARD domain-containing protein; apoptosis-associated speck-like protein containing a CARD
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (A00362-6). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASC/TMS1/PYCARD antigen affinity purified polyclonal antibody (Catalog # A00362-6) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASC/TMS1/PYCARD at approximately 24 kDa. The expected band size for ASC/TMS1/PYCARD is at 22 kDa.
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