Double-labeled immunofluorescence photomicrographs of paraffin-embedded sections of mouse colon. Green: E-Cadherin antibody (GTX100443) diluted at 1:500. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04). Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. The signal was developed using goat anti-mouse IgG antibody (Dylight594) (GTX213111-05). Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Glypican 1 antibody [N3C3] (GTX104557) detects Glypican 1 protein by flow cytometry analysis. Sample: A431 cell. Black: Unlabelled sample was used as a control. Red: Glypican 1 antibody [N3C3] (GTX104557) dilution: 1:50. Acquisition of 20,000 events were collected using the Rabbit IgG (DyLight488) (GTX113110-04) secondary antibody for FACS analysis.](https://www.genetex.com/upload/website/prouct_img/normal/GTX213110-04/GTX213110-04_42889_20190111_FACS_w_23060620_850.webp "Glypican 1 antibody [N3C3] (GTX104557) detects Glypican 1 protein by flow cytometry analysis. Sample: A431 cell. Black: Unlabelled sample was used as a control. Red: Glypican 1 antibody [N3C3] (GTX104557) dilution: 1:50. Acquisition of 20,000 events were collected using the Rabbit IgG (DyLight488) (GTX113110-04) secondary antibody for FACS analysis.")
![Double-labeled immunofluorescence photomicrographs of frozen sections of mouse brain. Green: Vimentin antibody (GTX100619) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04). Red: ME1 antibody [GT15611] (GTX632190) diluted at 1:200. The signal was developed using goat anti-mouse IgG antibody (Dylight594) (GTX213111-05). Blue: Nuclear staining with Hoechst 33342.](https://www.genetex.com/upload/website/prouct_img/normal/GTX213110-04/GTX213110-04_20150814_IHC-Fr_M_w_23060620_526.webp "Double-labeled immunofluorescence photomicrographs of frozen sections of mouse brain. Green: Vimentin antibody (GTX100619) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04). Red: ME1 antibody [GT15611] (GTX632190) diluted at 1:200. The signal was developed using goat anti-mouse IgG antibody (Dylight594) (GTX213111-05). Blue: Nuclear staining with Hoechst 33342.")
Double-labeled immunofluorescence photomicrographs of paraffin-embedded sections of mouse colon. Green: E-Cadherin antibody (GTX100443) diluted at 1:500. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04). Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. The signal was developed using goat anti-mouse IgG antibody (Dylight594) (GTX213111-05). Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Goat Anti-Rabbit IgG antibody (DyLight488)
GTX213110-04
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityRabbit
Overview
- SupplierGeneTex
- Product NameGoat Anti-Rabbit IgG antibody (DyLight488)
- Delivery Days Customer9
- Application Supplier NoteICC/IF: 1:100-1:2000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IHC-Wm: 1:100-1:500. FCM: 1:50-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration2 mg/ml
- ConjugateDyLight 488
- HostGoat
- IsotypeIgG
- ReactivityRabbit
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC41116161