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Goat anti-RBP1

EB06671
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetRBP1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-RBP1 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of U2OS cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5947
  • Target name
    RBP1
  • Target description
    retinol binding protein 1
  • Target synonyms
    cellular retinol binding protein 1; cellular retinol-binding protein I; CRABP-I; CRBP; CRBP1; CRBPI; RBPC; retinol-binding protein 1; retinol-binding protein 1, cellular
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_002890.2. Purification: Antigen affinity purified. Names and symbols: RBP1; CRBP; RBPC; CRBP1; CRABP-I; retinol binding protein 1, cellular; retinol-binding protein 1, cellular; CRBPI
  • Reactivity Supplier
    Human, Mouse, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RBP1 using anti-RBP1 antibody (A03820-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBP1 antigen affinity purified polyclonal antibody (Catalog # A03820-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RBP1 at approximately 16 kDa. The expected band size for RBP1 is at 16 kDa.
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