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ProductsBack/Goat anti-RFC4 Antibody, Affinity Purified

Goat anti-RFC4 Antibody, Affinity Purified

A300-144A
Bethyl Laboratories
ApplicationsWestern Blot
Product group Antibodies
TargetRFC4
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Goat anti-RFC4 Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5984
  • Target name
    RFC4
  • Target description
    replication factor C subunit 4
  • Target synonyms
    A1, MRMNS, RFC37, replication factor C subunit 4, A1 37 kDa subunit, RF-C 37 kDa subunit, RFC 37 kDa subunit, activator 1 37 kDa subunit, activator 1 subunit 4, replication factor C (activator 1) 4, 37kDa, replication factor C 37 kDa subunit
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDP35249
  • Protein Name
    Replication factor C subunit 4
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RFC4 using anti-RFC4 antibody (A07702-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFC4 antigen affinity purified polyclonal antibody (Catalog # A07702-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFC4 at approximately 37 kDa. The expected band size for RFC4 is at 40 kDa.
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