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Goat anti-RHBG Antibody

EB10279
Everest Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Mouse, Porcine, Rat
TargetRHBG
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-RHBG Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID57127
  • Target name
    RHBG
  • Target description
    Rh family B glycoprotein
  • Target synonyms
    SLC42A2, ammonium transporter Rh type B, Rhesus blood group, B glycoprotein, solute carrier family 42 member 2
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_065140.3. GeneIDs all Nonhuman: 58176 (mouse); 310625 (rat);. Purification: Antigen affinity purified. Names and symbols: RHBG; Rh family, B glycoprotein (gene/pseudogene); SLC42A2; Rh type B glycoprotein; Rhesus blood group, B glycoprotein
  • Reactivity
    Canine, Human, Mouse, Porcine, Rat
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of RHBG using anti-RHBG antibody (A05585-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RHBG antigen affinity purified polyclonal antibody (Catalog # A05585-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RHBG at approximately 47 kDa. The expected band size for RHBG is at 49 kDa.
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