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ProductsBack/Goat anti-SAP130 / SF3B3

Goat anti-SAP130 / SF3B3

EB05543
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSF3B3
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-SAP130 / SF3B3 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID23450
  • Target name
    SF3B3
  • Target description
    splicing factor 3b subunit 3
  • Target synonyms
    pre-mRNA splicing factor SF3b, 130 kDa subunit; RSE1; SAP 130; SAP130; SF3b130; spliceosome-associated protein 130; splicing factor 3B subunit 3; STAF130
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_036558.3. GeneIDs all Nonhuman: 101943 (mouse);. Purification: Antigen affinity purified. Names and symbols: SF3B3; SAP130; splicing factor 3b, subunit 3, 130kDa; RSE1; SF3B130; KIAA0017; STAF130; pre-mRNA splicing factor SF3b, 130 kDa subunit; spliceosome-associated protein 130; splicing factor 3b, subunit 3; SF3b130
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SF3B3 using anti-SF3B3 antibody (A08240-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SF3B3 antigen affinity purified polyclonal antibody (Catalog # A08240-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SF3B3 at approximately 136 kDa. The expected band size for SF3B3 is at 136 kDa.
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