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Goat anti-SEMA3E

EB06661
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSEMA3E
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-SEMA3E Antibody
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID9723
  • Target name
    SEMA3E
  • Target description
    semaphorin 3E
  • Target synonyms
    coll-5; M-SEMAH; M-SemaK; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E; SEMAH; semaphorin-3E
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_036563.1. Purification: Antigen affinity purified. Names and symbols: SEMA3E; SEMAH; coll-5; M-SEMAH; M-SemaK; KIAA0331; M-sema H; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3E
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SEMA3E using anti-SEMA3E antibody (A06937-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human U-87MG whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEMA3E antigen affinity purified polyclonal antibody (Catalog # A06937-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEMA3E at approximately 89KD. The expected band size for SEMA3E is at 89KD.
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