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ProductsBack/Goat anti-SENP6 / SUSP1 Antibody

Goat anti-SENP6 / SUSP1 Antibody

EB08330
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSENP6
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-SENP6 / SUSP1 Antibody
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID26054
  • Target name
    SENP6
  • Target description
    SUMO specific peptidase 6
  • Target synonyms
    SSP1, SUSP1, sentrin-specific protease 6, 2810017C20Rik, SUMO-1-specific protease 1, SUMO1/sentrin specific peptidase 6, SUMO1/sentrin specific protease 6, sentrin/SUMO-specific protease SENP6
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001093879.1; NP_056386.2. GeneIDs all Nonhuman: 215351 (mouse); 300860 (rat);. Purification: Antigen affinity purified. Names and symbols: SENP6; SUSP1; SUMO1/sentrin specific peptidase 6; RP1-134M13.1; FLJ11355; FLJ11887; KIAA0389; KIAA0797; SSP1; 2810017C20Rik; SUMO-1-specific protease; SUMO1/sentrin specific protease 6
  • Reactivity
    Human, Mouse, Rat
  • Reactivity Supplier
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SENP6 using anti-SENP6 antibody (A05088-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SENP6 antigen affinity purified polyclonal antibody (Catalog # A05088-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SENP6 at approximately 150KD. The expected band size for SENP6 is at 126KD.
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