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Goat anti-SLC12A1 / NKCC

EB09143
Everest Biotech
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetSLC12A1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-SLC12A1 / NKCC Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: This Antibody has been successfully used in IF on Mouse: Alshahrani S et al. (2015) PMID: 26400961.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID6557
  • Target name
    SLC12A1
  • Target description
    solute carrier family 12 member 1
  • Target synonyms
    BSC1; bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; kidney-specific Na-K-Cl symporter; Na-K-2Cl cotransporter; NKCC2; NKCC2A variant A; solute carrier family 12 (sodium/potassium/chloride transporter), member 1; solute carrier family 12 (sodium/potassium/chloride transporters), member 1; solute carrier family 12 member 1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000329.2. Purification: Antigen affinity purified. Names and symbols: SLC12A1; solute carrier family 12 (sodium/potassium/chloride transporters), member 1; BSC1; MGC48843; NKCC2; NKCC2A variant A; Na-K-2Cl cotransporter; sodium potassium chloride cotransporter 2
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Increased Slc12a1 expression in beta-cells and improved glucose disposal in Slc12a2 heterozygous mice. Alshahrani S et al., 2015 Dec, J Endocrinol
    Read more

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Figure 1. Western blot analysis of SLC12A1 using anti-SLC12A1 antibody (A01106). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey kidney tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC12A1 antigen affinity purified polyclonal antibody (Catalog # A01106) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC12A1 at approximately 150-290 kDa. The expected band size for SLC12A1 is at 121 kDa.
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