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ProductsBack/Goat anti-SLC6A4 / 5HTT / SERT

Goat anti-SLC6A4 / 5HTT / SERT

EB06743
Everest Biotech
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetSLC6A4
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-SLC6A4 / 5HTT / SERT Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in vesicles of HepG2 cells. Recommended concentration: 10microg/ml.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID6532
  • Target name
    SLC6A4
  • Target description
    solute carrier family 6 member 4
  • Target synonyms
    5HT transporter; 5HTT; 5-HTT; 5-HTTLPR; 5-hydroxytryptamine (serotonin) transporter; hSERT; HTT; Na+/Cl- dependent serotonin transporter; OCD1; serotonin transporter 1; SERT; SERT1; sodium-dependent serotonin transporter; solute carrier family 6 (neurotransmitter transporter), member 4; solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001036.1. Purification: Antigen affinity purified. Names and symbols: SLC6A4; SERT; solute carrier family 6 (neurotransmitter transporter, serotonin), member 4; HGNC:11050; 5-HTT; HTT; OCD1; hSERT; 5-hydroxytryptamine transporter; 5HT transporter; Na+/Cl- dependent serotonin transporter; serotonin transporter; sodium-depend
  • Reactivity Supplier
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC6A4 using anti-SLC6A4 antibody (PB9438). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human COLO-320 whole cell lysates. Lane 5: human K562 whole cell lysates, Lane 6: human U2OS whole cell lysates, Lane 7: human U-87MG whole cell lysates, Lane 8: rat heart tissue lysates, Lane 9: mouse heart tissue lysates, Lane 10: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC6A4 antigen affinity purified polyclonal antibody (Catalog # PB9438) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC6A4 at approximately 90KD. The expected band size for SLC6A4 is at 70KD.
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