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ProductsBack/Goat anti-SLITRK1

Goat anti-SLITRK1

EB07047
Everest Biotech
ApplicationsELISA
Product group Antibodies
TargetSLITRK1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-SLITRK1 Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA
  • Applications Supplier
    Pep-ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID114798
  • Target name
    SLITRK1
  • Target description
    SLIT and NTRK like family member 1
  • Target synonyms
    leucine-rich repeat-containing protein 12; LRRC12; SLIT and NTRK-like protein 1; slit and trk like gene 1; TTM
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_443142.1. Purification: Antigen affinity purified. Names and symbols: SLITRK1; SLIT and NTRK-like family, member 1; HGNC:20297; KIAA0918; KIAA1910; LRRC12; RP11-395N17.1; leucine rich repeat containing 12; slit and trk like 1 protein; slit and trk like gene 1; FLJ54428
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLITRK1 using anti-SLITRK1 antibody (A07507-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human U2OS whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLITRK1 antigen affinity purified polyclonal antibody (Catalog # A07507-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLITRK1 at approximately 55 kDa. The expected band size for SLITRK1 is at 78 kDa.
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