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Goat anti-Sorbitol Dehydrogenase

EB06601
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSORD
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Sorbitol Dehydrogenase Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID6652
  • Target name
    SORD
  • Target description
    sorbitol dehydrogenase
  • Target synonyms
    HEL-S-95n, HMNR8, RDH, SDH, SORD1, SORDD, XDH, sorbitol dehydrogenase, (R,R)-butanediol dehydrogenase, L-iditol 2-dehydrogenase, epididymis secretory sperm binding protein Li 95n, polyol dehydrogenase, ribitol dehydrogenase, xylitol dehydrogenase
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_003095.2. Purification: Antigen affinity purified. Names and symbols: sorbitol dehydrogenase; SORD; SORD1
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Niimi N, Yako H, Takaku S, et al. A spontaneously immortalized Schwann cell line from aldose reductase-deficient mice as a useful tool for studying polyol pathway and aldehyde metabolism. J Neurochem. 2018,144(6):710-722. doi: 10.1111/jnc.14277
    Read this paper

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Figure 1. Western blot analysis of Sorbitol Dehydrogenase/ SORD using anti-Sorbitol Dehydrogenase/ SORD antibody (A07851-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U937 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: monkey kidney tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse liver tissue lysates, Lane 9: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sorbitol Dehydrogenase/ SORD antigen affinity purified polyclonal antibody (Catalog # A07851-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sorbitol Dehydrogenase/ SORD at approximately 43KD. The expected band size for Sorbitol Dehydrogenase/ SORD is at 38KD.
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